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first letter of test name, or select test group, to review specifications: |
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| HIV-1 RNA 3.0 bDNA Assay |
|
| Alternate Names: | HIV Viral Load, Branch DNA for HIV Viral Load |
| Performed: | 1st shift: Set-Up - Wednesday afternoon; Resulted - Thursday afternoon Test performed at Cape Fear Laboratory ( Runs are batched one time per week ; Samples must be received by 1000 Wednesday to be included in the weekly run). |
| Methodology: | Branched DNA Signal Amplification (bDNA) |
| Turnaround Time: | ~ 1 week |
| Container / Specimen Requirements |
Plastic Screw-Cap Vial |
| Specimen Requirements : |
2.5mL frozen EDTA plasma collected in PPT vacutainer or lavender-top
(EDTA) tube.
Plasma only; NO SERUM Plasma: Collect blood in sterile tubes containing EDTA anticoagulant (Lavender-Top) or PPT (White-Top) tube. Blood collected in tubes containing ACD anticoagulant are acceptable, but will yield results approximately 15% lower when compared to EDTA tubes due to the dilution effect of the 1.5mL of anticoagulant used in the tube. Separate plasma from the cells by centrifugation within 4hours after collection, transfer the plasma to a separate plastic screw-cap vial and freeze. Frozen PPT tubes are unacceptable. |
| Minimum Volume: | 1.0 mL |
| Special Handling or Timing of Specimen: | Specimen Stability Room Temperature: up to 4 hours Frozen: at -60 degrees to -80 degrees C until testing |
| Preparation of Patient: | None |
| Reference Range: | < 75 copies / mL < 1.88 log copies / mL |
| Causes for Specimen Rejection: | Improper labeling.
Received room temperature; heparinized plasma; refrigerated; serum; or frozen PPT tubes |
| CareManager Test Group: CareManager Name/Alias: |
CLAB HIV-1 RNA bDNA Assay |
| Specimen Labeling: | Click here for policy |
2131 South 17th Street Wilmington, North Carolina 28401 910.343.7000
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